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KMID : 0620920010330030145
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Experimental & Molecular Medicine
2001 Volume.33 No. 3 p.145 ~ p.149
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Development of a packaging cell line for propagation of replication-deficient adenovirus vector
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Je Ho Lee/Jong Sik Kim
Seung Hoon Lee/Yong Suk Cho/Kyoung Sook Park/Young Ho Kim/Je Ho Lee
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Abstract
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A human embryonic kidney cell line 293 is widely used for adenovirus production and propagation. With this cell line, however, replication-competent virus (RCV) is frequently generated, especially during large-scale production and successive propagation because 293 cells contain not only E1 gene but also non-E1 adenovirus gene. Homologous recombination between non-E1 region of 293 genomic DNA and its homologous region in the recombinant adenoviral vector generate RCV. To overcome this problem, we developed a new packaging cell line, Hela-E1, which contains minimum E1 region and from which non-E1 adenoviral region that is homologous with recombinant adenovirus vector was excluded. No RCV was detected during adenovirus propagation in Hela-E1 compared to in 293. In addition, adenovirus-p53 produced in HeLa-E1 was able to overexpress p53 protein when introduced into an ovarian cancer cell line, SKOV3. These results may have a significant impact on the development of packaging cell lines for replication-deficient adenovirus production.
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KEYWORD
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adenovirus, replication competent virus, packaging cell line, HeLa-E1 cell,
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